CRISPincette™ LUNG
CRISPincette™ LUNG
PRODUCT SPECIFICATION
Input DNA
FFPE, Tissue gDNA ≥ 10 ng * 2 SET / cfDNA ≥ 3 ng * 2 SET
Target enrichment
Amplicon method
Platform
Illumina MiSeq or MiSeq DX
* MiSeq Reagent Micro kit v2 or kit v2
Target region
Including 10 target regions associated with NSCLC
Variant types
Indel, SNV
Analysis solution
In-house
PERFORMANCE EVALUATION
KEY FEATURES
Diagnostic kit based on NGS
NGS diagnostic kit to detect major
NSCLC-related mutant DNA
Convenience of diagnosis
Enhanced diagnostic convenience using
gDNA in Tissue, FFPE
CRISPR/Cas9 system
Amplify mutant DNA after cutting
wild-type DNA
High sensitivity
Enhanced detection limits using gcCas9
Reasonable cost
Compared to conventional NGS diagnostic kits,
a greater number of samples can be analyzed in a single run
All-in-one platform
Integrated systems from diagnosis
to analysis at once
PROCESS
- 01 cfDNA prep
gDNA prep - 02 Target Amplification
(1st PCR) - 03 In vitro Cleavage
- 04 Library Clean up
- 05 Index
(2nd PCR) - 06 Pooling
- 07 NGS & Analysis
Blood
Tissue / FFPE
cfDNA prep /
gDNA prep
Target Amplification (1st PCR)
In vitro Cleavage
Library Clean up Removes cut wild-type DNA
Index (2nd PCR)
NGS & Analysis